Journal: Discover Oncology

Article Title: Integrated WGCNA retrieval of T-cell exhaustion genes related to radiosensitivity in locally advanced cervical cancer and prediction of immunotherapy efficacy

doi: 10.1007/s12672-025-03673-y

Figure Lengend Snippet: IHC assay results of the protein expression levels of APIBEC3H, CCR7, PILRA, and SLC15A3 in LACC patients

Article Snippet: Subsequently, the sections were incubated at 4 °C overnight with the corresponding primary antibody against APOBEC3H (1:200, PA5-54426, Invitrogen, USA), CCR7 (1:100, 25898-1-AP, Proteintech, China), PLRA (1:100, PA5-147474, Invitrogen, USA), SLC15A3 (1:100, PA5-61614, Invitrogen, USA).

Techniques: Expressing

Journal: PLoS ONE

Article Title: CCL21/CCR7 Enhances the Proliferation, Migration, and Invasion of Human Bladder Cancer T24 Cells

doi: 10.1371/journal.pone.0119506

Figure Lengend Snippet: Effect of CCL21 on T24 cell proliferation.

Article Snippet: CCL21 recombinant human protein was purchased from Perprotech (Rocky Hill, NJ, USA) and polyclonal rabbit anti-human CCR7 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (Wuhan, China).

Techniques: Control

Journal: PLoS ONE

Article Title: CCL21/CCR7 Enhances the Proliferation, Migration, and Invasion of Human Bladder Cancer T24 Cells

doi: 10.1371/journal.pone.0119506

Figure Lengend Snippet: The assay was performed using a transwell chamber coated with 30 μl of Matrigel mixture solution with five different groups consisting of vehicle control, CCR7 antibody 20 ng/ml plus CCL21 50 ng/ml, CCL21 50 ng/ml, CCL21 100 ng/ml, and CCL21 200 ng/ml. The data present Mean ± SD from four independent experiments.

Article Snippet: CCL21 recombinant human protein was purchased from Perprotech (Rocky Hill, NJ, USA) and polyclonal rabbit anti-human CCR7 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (Wuhan, China).

Techniques: Control

Journal: PLoS ONE

Article Title: CCL21/CCR7 Enhances the Proliferation, Migration, and Invasion of Human Bladder Cancer T24 Cells

doi: 10.1371/journal.pone.0119506

Figure Lengend Snippet: Lane 1: T24 cells treated with vehicle control; Lane 2: T24 cells treated with CCR7 antibody 20 ng/ml plus CCL21 50 ng/ml; Lane 3: T24 cells treated with 50 ng/ml CCL21; Lane 4: T24 cells treated with 100 ng/ml CCL21; and Lane 5: T24 cells treated with 200 ng/ml CCL21. Cell lysates were prepared and run on 5–15% SDS-PAGE gels following by a Western blot analysis. Beta-actin was used as a loading control. The blots shown are representative of three independent experiments.

Article Snippet: CCL21 recombinant human protein was purchased from Perprotech (Rocky Hill, NJ, USA) and polyclonal rabbit anti-human CCR7 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (Wuhan, China).

Techniques: Control, SDS Page, Western Blot

Journal: PLoS ONE

Article Title: CCL21/CCR7 Enhances the Proliferation, Migration, and Invasion of Human Bladder Cancer T24 Cells

doi: 10.1371/journal.pone.0119506

Figure Lengend Snippet: Effects of CCL21/CCR7 on MMP-2 and MMP-9 expression as represented via OD values.

Article Snippet: CCL21 recombinant human protein was purchased from Perprotech (Rocky Hill, NJ, USA) and polyclonal rabbit anti-human CCR7 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (Wuhan, China).

Techniques: Expressing, Control

Journal: PLoS ONE

Article Title: CCL21/CCR7 Enhances the Proliferation, Migration, and Invasion of Human Bladder Cancer T24 Cells

doi: 10.1371/journal.pone.0119506

Figure Lengend Snippet: T24 cells were treated with vehicle control, CCR7 antibody 20 ng/ml plus CCL21 50 ng/ml, CCL21 50 ng/ml, CCL21 100 ng/ml, and CCL21 200 ng/ml. The values (OD) present Mean ± SD from three independent experiments.

Article Snippet: CCL21 recombinant human protein was purchased from Perprotech (Rocky Hill, NJ, USA) and polyclonal rabbit anti-human CCR7 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (Wuhan, China).

Techniques: Control

Journal: PLoS ONE

Article Title: CCL21/CCR7 Enhances the Proliferation, Migration, and Invasion of Human Bladder Cancer T24 Cells

doi: 10.1371/journal.pone.0119506

Figure Lengend Snippet: Antiapoptotic effects of CCL21/CCR7 on T24.

Article Snippet: CCL21 recombinant human protein was purchased from Perprotech (Rocky Hill, NJ, USA) and polyclonal rabbit anti-human CCR7 antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (Wuhan, China).

Techniques: Control